Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

RanBPM, a novel interaction partner of the brain-specific protein p42IP4/centaurin alpha-1.

Haase A, Nordmann C, Sedehizade F, Borrmann C, Reiser G

The protein p42(IP4) (aka Centaurin alpha-1) is highly enriched in the brain and has specific binding sites for the membrane lipid phosphatidylinositol 3,4,5-trisphosphate and the soluble messenger inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P(4); IP4). p42(IP4) shuttles between plasma membrane, cytosol and cell nucleus. However, the molecular function of p42(IP4) is still largely unclear. Here, we report a novel interaction partner for p42(IP4), Ran ... [more]

J. Neurochem. Jun. 01, 2008; 105(6);2237-48 [Pubmed: 18298663]

Throughput

  • Low Throughput

Curated By

  • BioGRID