BAIT

ADAP1

Centa1, p42IP4
ArfGAP with dual PH domains 1
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus
PREY

RANBP9

RGD1561940, RanBPM
RAN binding protein 9
GO Process (0)
GO Function (2)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RanBPM, a novel interaction partner of the brain-specific protein p42IP4/centaurin alpha-1.

Haase A, Nordmann C, Sedehizade F, Borrmann C, Reiser G

The protein p42(IP4) (aka Centaurin alpha-1) is highly enriched in the brain and has specific binding sites for the membrane lipid phosphatidylinositol 3,4,5-trisphosphate and the soluble messenger inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P(4); IP4). p42(IP4) shuttles between plasma membrane, cytosol and cell nucleus. However, the molecular function of p42(IP4) is still largely unclear. Here, we report a novel interaction partner for p42(IP4), Ran ... [more]

J. Neurochem. Jun. 01, 2008; 105(6);2237-48 [Pubmed: 18298663]

Throughput

  • Low Throughput

Curated By

  • BioGRID