Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

The proteomic investigation of chromatin functional domains reveals novel synergisms among distinct heterochromatin components.

Soldi M, Bonaldi T

Chromatin is a highly dynamic, well-structured nucleoprotein complex of DNA and proteins that controls virtually all DNA transactions. Chromatin dynamicity is regulated at specific loci by the presence of various associated proteins, histones, post-translational modifications, histone variants, and DNA methylation. Until now the characterization of the proteomic component of chromatin domains has been held back by the challenge of enriching ... [more]

Mol. Cell Proteomics Mar. 01, 2013; 12(3);764-80 [Pubmed: 23319141]

Throughput

  • Low Throughput

Additional Notes

  • ChIP analysis; WICH complex enriched in heterochromatin regions marked by H3K9me3 by MS

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HIST1H3A BAZ1B
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High8.47BioGRID
2990216
HIST1H3A BAZ1B
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID