BAIT

COPS2

ALIEN, CSN2, SGN2, TRIP15
COP9 signalosome subunit 2
UPS Project
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

5830415F09RIK

AV014846, Nap1, RP23-423O7.7
RIKEN cDNA 5830415F09 gene
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The corepressor activity of Alien is controlled by CREB-binding protein/p300.

Eckey M, Kraft F, Kob R, Escher N, Asim M, Fischer H, Fritsche MK, Melle C, Baniahmad A

The regulation of gene repression by corepressors is a controlled process. Surface-enhanced laser desorption ionization MS proteomic analysis and a yeast two-hybrid screen showed independently that the corepressor Alien interacts with the CREB-binding protein (CBP) coactivator. This interaction was further confirmed by coimmunoprecipitation and glutathione S-transferase pull-down experiments, suggesting that Alien interacts in vivo and in vitro with the histone ... [more]

FEBS J. Apr. 01, 2013; 280(8);1861-8 [Pubmed: 23441852]

Throughput

  • Low Throughput

Curated By

  • BioGRID