Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The sarcomeric Z-disc component myopodin is a multiadapter protein that interacts with filamin and alpha-actinin.

Linnemann A, van der Ven PF, Vakeel P, Albinus B, Simonis D, Bendas G, Schenk JA, Micheel B, Kley RA, Fuerst DO

Here we introduce myopodin as a novel filamin C binding partner. Corroborative yeast two-hybrid and biochemical analyses indicate that the central part of myopodin that shows high homology to the closely related protein synaptopodin and that is common to all its currently known or predicted variants interacts with filamin C immunoglobulin-like domains 20-21. A detailed characterization of the previously described ... [more]

Eur. J. Cell Biol. Sep. 01, 2010; 89(9);681-92 [Pubmed: 20554076]

Throughput

Low Throughput

Additional Notes

Figure 1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FLNC SYNPO2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Linnemann A (2010)	Low	-	BioGRID	949046

Curated By

BioGRID

Interaction Summary

FLNC

Gene Ontology Biological Process

Gene Ontology Molecular Function

ankyrin binding [IPI]cytoskeletal protein binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

SYNPO2

Gene Ontology Molecular Function

14-3-3 protein binding [ISS]muscle alpha-actinin binding [ISS]protein binding [IPI]