BAIT

FLNC

ABP-280, ABP280A, ABPA, ABPL, FLN2, MFM5, MPD4
filamin C, gamma
GO Process (1)
GO Function (3)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

XIRP1

CMYA1, Xin
xin actin-binding repeat containing 1
GO Process (0)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP.

van der Ven PF, Ehler E, Vakeel P, Eulitz S, Schenk JA, Milting H, Micheel B, Fuerst DO

Filamin c is the predominantly expressed filamin isoform in striated muscles. It is localized in myofibrillar Z-discs, where it binds FATZ and myotilin, and in myotendinous junctions and intercalated discs. Here, we identify Xin, the protein encoded by the human gene 'cardiomyopathy associated 1' (CMYA1) as filamin c binding partner at these specialized structures where the ends of myofibrils are ... [more]

Exp. Cell Res. Jul. 01, 2006; 312(11);2154-67 [Pubmed: 16631741]

Throughput

  • Low Throughput

Additional Notes

  • Figure 1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FLNC XIRP1
Far Western
Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Low-BioGRID
949548
XIRP1 FLNC
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
949010

Curated By

  • BioGRID