Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP.

van der Ven PF, Ehler E, Vakeel P, Eulitz S, Schenk JA, Milting H, Micheel B, Fuerst DO

Filamin c is the predominantly expressed filamin isoform in striated muscles. It is localized in myofibrillar Z-discs, where it binds FATZ and myotilin, and in myotendinous junctions and intercalated discs. Here, we identify Xin, the protein encoded by the human gene 'cardiomyopathy associated 1' (CMYA1) as filamin c binding partner at these specialized structures where the ends of myofibrils are ... [more]

Exp. Cell Res. Jul. 01, 2006; 312(11);2154-67 [Pubmed: 16631741]

Throughput

Low Throughput

Additional Notes

Figure 1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FLNC XIRP1	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	van der Ven PF (2006)	Low	-	BioGRID	949548
XIRP1 FLNC	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kley RA (2013)	Low	-	BioGRID	949010

Curated By

BioGRID

Interaction Summary

FLNC

Gene Ontology Biological Process

Gene Ontology Molecular Function

ankyrin binding [IPI]cytoskeletal protein binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

XIRP1

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Two-hybrid