PREY

UBR4

RBAF600, ZUBR1, p600, RP5-1126H10.1
ubiquitin protein ligase E3 component n-recognin 4
GO Process (1)
GO Function (2)
GO Component (4)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling.

Morrison J, Laurent-Rolle M, Maestre AM, Rajsbaum R, Pisanelli G, Simon V, Mulder LC, Fernandez-Sesma A, Garcia-Sastre A

An estimated 50 million dengue virus (DENV) infections occur annually and more than forty percent of the human population is currently at risk of developing dengue fever (DF) or dengue hemorrhagic fever (DHF). Despite the prevalence and potential severity of DF and DHF, there are no approved vaccines or antiviral therapeutics available. An improved understanding of DENV immune evasion is ... [more]

PLoS Pathog. Mar. 01, 2013; 9(3);e1003265 [Pubmed: 23555265]

Throughput

  • Low Throughput

Additional Notes

  • in the presence of the DENV NS5 protein

Curated By

  • BioGRID