BCL11A
Gene Ontology Biological Process
- negative regulation of axon extension [ISS]
- negative regulation of collateral sprouting [IMP]
- negative regulation of dendrite development [IMP]
- negative regulation of neuron projection development [IDA]
- negative regulation of protein homooligomerization [IC]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of collateral sprouting [IMP]
- positive regulation of neuron projection development [IDA]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- protein sumoylation [ISS]
- regulation of dendrite development [IMP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [TAS]
NR2F2
Gene Ontology Biological Process
- intracellular receptor signaling pathway [IDA, TAS]
- lipid metabolic process [TAS]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of endothelial cell migration [IMP]
- negative regulation of endothelial cell proliferation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription involved in lymphatic endothelial cell fate commitment [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- retinoic acid binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IC, IDA]
- transcription corepressor activity [TAS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- retinoic acid binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IC, IDA]
- transcription corepressor activity [TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors.
Despite their physiological importance, selective interactions between nuclear receptors (NRs) and their cofactors are poorly understood. Here, we describe a novel signature motif (F/YSXXLXXL/Y) in the developmental regulator BCL11A that facilitates its selective interaction with members of the NR2E/F subfamily. Two copies of this motif (named here as RID1 and RID2) permit BCL11A to bind COUP-TFs (NR2F1;NR2F2;NR2F6) and Tailless/TLX (NR2E1), ... [more]
Throughput
- Low Throughput
Additional Notes
- source of proteins unclear
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NR2F2 BCL11A | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 950519 | |
| BCL11A NR2F2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 950516 | |
| NR2F2 BCL11A | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 950518 |
Curated By
- BioGRID