Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

SOCS3 regulates BAFF in human enterocytes under ribosomal stress.

Do KH, Choi HJ, Kim J, Park SH, Kim KH, Moon Y

Although the activation of B cells in the gastrointestinal tract is of great importance in the context of immunity to pathogens and mucosal inflammatory diseases, little is known about the mechanisms responsible for the local activation of B cells in the subepithelial area of the intestine. Epithelium-derived BAFF is the major modulator of B cell development and Ig class switching. ... [more]

J. Immunol. Jun. 15, 2013; 190(12);6501-10 [Pubmed: 23686487]

Throughput

  • Low Throughput

Curated By

  • BioGRID