An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Suppressor of Cytokine Signaling (SOCS) 5 utilises distinct domains for regulation of JAK1 and interaction with the adaptor protein Shc-1.

Linossi EM, Chandrashekaran IR, Kolesnik TB, Murphy JM, Webb AI, Willson TA, Kedzierski L, Bullock AN, Babon JJ, Norton RS, Nicola NA, Nicholson SE

Suppressor of Cytokine Signaling (SOCS)5 is thought to act as a tumour suppressor through negative regulation of JAK/STAT and epidermal growth factor (EGF) signaling. However, the mechanism/s by which SOCS5 acts on these two distinct pathways is unclear. We show for the first time that SOCS5 can interact directly with JAK via a unique, conserved region in its N-terminus, which ... [more]

PLoS ONE Aug. 31, 2013; 8(8);e70536 [Pubmed: 23990909]

Throughput

Low Throughput

Additional Notes

Actually a pulldown-MS, which is a reconstituted complex

Curated By

BioGRID

Interaction Summary

SOCS4

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein kinase inhibitor activity [IBA]

Gene Ontology Cellular Component

CUL5