An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interaction between salt-inducible kinase 2 (SIK2) and p97/valosin-containing protein (VCP) regulates endoplasmic reticulum (ER)-associated protein degradation in mammalian cells.

Yang FC, Lin YH, Chen WH, Huang JY, Chang HY, Su SH, Wang HT, Chiang CY, Hsu PH, Tsai MD, Tan BC, Lee SC

Salt-inducible kinase 2 (SIK2) is an important regulator of cAMP response element-binding protein-mediated gene expression in various cell types and is the only AMP-activated protein kinase family member known to interact with the p97/valosin-containing protein (VCP) ATPase. Previously, we have demonstrated that SIK2 can regulate autophagy when proteasomal function is compromised. Here we report that physical and functional interactions between ... [more]

J. Biol. Chem. Nov. 22, 2013; 288(47);33861-72 [Pubmed: 24129571]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SIK2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]magnesium ion binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

HSPA4