Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ubp-M serine 552 phosphorylation by cyclin-dependent kinase 1 regulates cell cycle progression.

Xu Y, Yang H, Joo HY, Yu JH, Smith AD, Schneider D, Chow LT, Renfrow M, Wang H

In eukaryotic cells, genomic DNA is organized into a chromatin structure, which not only serves as the template for DNA-based nuclear processes, but also as a platform integrating intracellular and extracellular signals. Although much effort has been spent to characterize chromatin modifying/remodeling activities, little is known about cell signaling pathways targeting these chromatin modulators. Here, we report that cyclin-dependent kinase ... [more]

Cell Cycle Oct. 01, 2013; 12(19);3219-27 [Pubmed: 24013421]

Throughput

  • Low Throughput

Curated By

  • BioGRID