An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The E3 Ligase CHIP Mediates Ubiquitination and Degradation of Mixed-Lineage Kinase 3.

Blessing NA, Brockman AL, Chadee DN

Mixed-lineage kinase 3 (MLK3) activates mitogen-activated protein kinase (MAPK) signaling pathways and has important functions in migration, invasion, proliferation, tumorigenesis, and apoptosis. We investigated the role of the E3 ligase carboxyl terminus of Hsc70-interacting protein (CHIP) in the regulation of MLK3 protein levels. We show that CHIP interacts with MLK3 and, together with the E2 ubiquitin-conjugating enzyme UbcH5 (UbcH5a, -b, ... [more]

Mol. Cell. Biol. Aug. 15, 2014; 34(16);3132-43 [Pubmed: 24912674]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MAP3K11

Gene Ontology Biological Process

Gene Ontology Molecular Function

JUN kinase kinase kinase activity [ISS]identical protein binding [IPI]mitogen-activated protein kinase kinase kinase binding [IPI]protein binding [IPI]protein homodimerization activity [IPI]protein kinase activity [TAS]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

HSPA4