BAIT

DSC2

YOL073C, YOL073C

Multi-transmembrane subunit of the DSC ubiquitin ligase complex; similar in sequence to rhomboid pseudoproteases Der1p and UBAC2 that function in ERAD; ortholog of fission yeast dsc2

UPS Project

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

VPS27

DID7, GRD11, SSV17, VPL23, VPT27, ESCRT-0 subunit protein VPS27, L000002790, YNR006W

Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)

UPS Project

GO Process (3)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

late endosome to vacuole transport [IMP]

protein retention in Golgi apparatus [IMP]

protein targeting to vacuole [IMP, IPI]

Gene Ontology Molecular Function

phosphatidylinositol-3-phosphate binding [IDA]
ubiquitin binding [IDA]

Gene Ontology Cellular Component

ESCRT-0 complex [IPI]

endosome [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Identification of Candidate Substrates for the Golgi Tul1 E3 Ligase Using Quantitative diGly Proteomics in Yeast.

Tong Z, Kim MS, Pandey A, Espenshade PJ

Maintenance of protein homeostasis is essential for cellular survival. Central to this regulation are mechanisms of protein quality control in which misfolded proteins are recognized and degraded by the ubiquitin-proteasome system. One well-studied protein quality control pathway requires ER resident, multi-subunit E3 ubiquitin ligases that function in ER-associated degradation (ERAD). Using fission yeast, our lab identified the Golgi Dsc E3 ... [more]

Mol. Cell Proteomics Jul. 30, 2014; 0(0); [Pubmed: 25078903]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: heat sensitivity (APO:0000147)

Additional Notes

37 deg C

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VPS27 DSC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.2214	BioGRID	411683
VPS27 DSC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2372	BioGRID	2176527
DSC2 VPS27	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1916	BioGRID	2179660

Curated By

BioGRID

Interaction Summary