BAIT

DSC3

YOR223W, YOR223W

Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3

UPS Project

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

VPS25

VPL12, VPT25, ESCRT-II subunit protein VPS25, YJR102C

Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome

GO Process (3)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]

protein targeting to vacuole [IMP, IPI]

ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IC]

Gene Ontology Molecular Function

structural molecule activity [IDA, IMP]

Gene Ontology Cellular Component

ESCRT II complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Identification of Candidate Substrates for the Golgi Tul1 E3 Ligase Using Quantitative diGly Proteomics in Yeast.

Tong Z, Kim MS, Pandey A, Espenshade PJ

Maintenance of protein homeostasis is essential for cellular survival. Central to this regulation are mechanisms of protein quality control in which misfolded proteins are recognized and degraded by the ubiquitin-proteasome system. One well-studied protein quality control pathway requires ER resident, multi-subunit E3 ubiquitin ligases that function in ER-associated degradation (ERAD). Using fission yeast, our lab identified the Golgi Dsc E3 ... [more]

Mol. Cell Proteomics Jul. 30, 2014; 0(0); [Pubmed: 25078903]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Additional Notes

37 deg C

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VPS25 DSC3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.171	BioGRID	392679
VPS25 DSC3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2197	BioGRID	2140082

Curated By

BioGRID

Interaction Summary