Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Degradation of Tiam1 by Casein Kinase 1 and the SCFβTrCP Ubiquitin Ligase Controls the Duration of mTOR-S6K signaling.

Magliozzi R, Kim J, Low TY, Heck AJ, Guardavaccaro D

T-cell lymphoma invasion and metastasis 1 (Tiam1) is a guanine nucleotide exchange factor that specifically controls the activity of the small GTPase Rac, a key regulator of cell adhesion, proliferation and survival. Here, we report that in response to mitogens, Tiam1 is degraded by the ubiquitin-proteasome system via the SCFbetaTrCP ubiquitin ligase. Mitogenic stimulation triggers the binding of Tiam1 to ... [more]

J. Biol. Chem. Aug. 14, 2014; 0(0); [Pubmed: 25124033]

Throughput

  • Low Throughput

Curated By

  • BioGRID