Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Characterization of a novel binding partner of the melanocortin-4 receptor: attractin-like protein.

Haqq AM, Rene P, Kishi T, Khong K, Lee CE, Liu H, Friedman JM, Elmquist JK, Cone RD

The gene dosage effect of the MC4-R (melanocortin 4 receptor) on obesity suggests that regulation of MC4-R expression and function is critically important to the central control of energy homoeostasis. In order to identify putative MC4-R regulatory proteins, we performed a yeast two-hybrid screen of a mouse brain cDNA library using the mouse MC4-R intracellular tail (residues 303-332) as bait. ... [more]

Biochem. J. Dec. 15, 2003; 376(0);595-605 [Pubmed: 14531729]

Throughput

  • Low Throughput

Curated By

  • BioGRID