An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interaction of G-protein Î²Î³ complex with chromatin modulates GPCR-dependent gene regulation.

Bhatnagar A, Unal H, Jagannathan R, Kaveti S, Duan ZH, Yong S, Vasanji A, Kinter M, Desnoyer R, Karnik SS

Heterotrimeric G-protein signal transduction initiated by G-protein-coupled receptors (GPCRs) in the plasma membrane is thought to propagate through protein-protein interactions of subunits, GÎ± and GÎ²Î³ in the cytosol. In this study, we show novel nuclear functions of GÎ²Î³ through demonstrating interaction of GÎ²(2) with integral components of chromatin and effects of GÎ²(2) depletion on global gene expression. Agonist activation of ... [more]

PLoS ONE Jan. 18, 2013; 8(1);e52689 [Pubmed: 23326349]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GNB2

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [TAS]GTPase binding [IPI]protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component

HIST2H2BE