BAIT

MCM7

CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104
minichromosome maintenance complex component 7
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein-protein interaction of FHL2, a LIM domain protein preferentially expressed in human heart, with hCDC47.

Chan KK, Tsui SK, Ngai SM, Lee SM, Kotaka M, Waye MM, Lee CY, Fung KP

In the yeast two-hybrid library screening, the heart-specific FHL2 protein was found to interact with hCDC47. In vitro interaction study between FHL2 protein and hCDC47 was demonstrated. From the results of domain studies by the yeast two-hybrid assay, the second and third LIM domains in conjunction with the first half LIM domain of FHL2 were identified to be important in ... [more]

J. Cell. Biochem. Jan. 01, 2000; 76(3);499-508 [Pubmed: 10649446]

Throughput

  • Low Throughput

Curated By

  • BioGRID