Mitosis is an essential biological function that requires the spatial and temporal coordination of hundreds of proteins to ensure the correct assembly, function, and regulation of molecular complexes driving human cell division. Mitotic spindle assembly proteins are critical for the establishment of the bipolar spindle and subsequent segregation of chromosomes, thereby maintaining genomic stability in healthy cells. Dysregulation of these genes can cause defective spindle assembly, resulting in chromosomal instability, aneuploidy, and increased cancer or developmental disease risk.

The MitoCheck consortium carried out genome-wide phenotypic profiling to identify human genes necessary for cell cycle-related functions such as chromosome segregation, cellular division and survival (PMIDs 20360735, 20360068). A core set of mitotic spindle assembly genes and their corresponding mitotic phenotypes were obtained from these published results. The list was further refined in consultation with Jean-Karim Heriche (The MitoCheck database v2.1) (PMID: 30202089) and domain experts from Sabine Petry’s lab.

The corresponding molecular interactions for these genes were manually curated from the scientific literature by BioGRID curators to produce a spindle assembly specific dataset. This project page allows users to explore this gene set and their MitoCheck assigned phenotypes, as well as any relevant protein interactions, post-translational modifications (PTMs), chemical interactions, and BioGRID ORCS CRISPR screen phenotypes.

This curation project is on-going and will be updated each month. If you notice errors or missing interactions/publications in this dataset, or otherwise wish to contribute to this project, please contact us at support@thebiogrid.org. All mitotic spindle assembly database records are freely available for download from links within this project.