SGS3
Gene Ontology Biological Process
RDR6
Gene Ontology Biological Process
- RNA interference [IMP]
- carpel development [IGI]
- defense response to virus [IMP]
- gene silencing by RNA [IMP]
- gynoecium development [IMP]
- leaf development [IGI]
- posttranscriptional gene silencing [TAS]
- production of miRNAs involved in gene silencing by miRNA [IMP]
- production of siRNA involved in RNA interference [TAS]
- production of ta-siRNAs involved in RNA interference [IMP]
- recognition of pollen [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies.
Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. Interestingly, the granules containing SGS3 and RDR6 (named SGS3/RDR6-bodies) were distinct from the processing bodies where mRNAs ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RDR6 SGS3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1174448 |
Curated By
- BioGRID