BAIT

CINV1

F15O4.33, cytosolic invertase 1, AT1G35580
alkaline/neutral invertase CINV1
Arabidopsis thaliana (Columbia)
PREY

GRF10

14-3-3 PROTEIN G-BOX FACTOR14 EPSILON, 14-3-3EPSILON, GF14 EPSILON, general regulatory factor 10, AT1G22300
14-3-3-like protein GF14 epsilon
GO Process (2)
GO Function (2)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Light modulated activity of root alkaline/neutral invertase involves the interaction with 14-3-3 proteins.

Gao J, van Kleeff PJ, Oecking C, Li KW, Erban A, Kopka J, Hincha DK, de Boer AH

Alkaline/neutral invertases (A/N-Invs) are now recognized as essential proteins in plant life. They catalyze the irreversible breakdown of sucrose into glucose and fructose and thus supply the cells with energy as well as signaling molecules. In this study we report on a mechanism that affects the activity of the cytosolic invertase AtCINV1 (At-A/N-InvG or AT1G35580). We demonstrate that Ser547 at ... [more]

Plant J. Dec. 01, 2014; 80(5);785-96 [Pubmed: 25256212]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GRF10 CINV1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
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Curated By

  • BioGRID