Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.

Liu X, Reitsma JM, Mamrosh JL, Zhang Y, Straube R, Deshaies RJ

Skp1⋅Cul1⋅F-box (SCF) ubiquitin ligase assembly is regulated by the interplay of substrate binding, reversible Nedd8 conjugation on Cul1, and the F-box protein (FBP) exchange factors Cand1 and Cand2. Detailed investigations into SCF assembly and function in reconstituted systems and Cand1/2 knockout cells informed the development of a mathematical model for how dynamical assembly of SCF complexes is controlled and how this ... [more]

Mol. Cell Dec. 01, 2017; 69(5);773-786.e6 [Pubmed: 29499133]

Throughput

  • Low Throughput

Additional Notes

  • Interaction is dependent on RBX1-CUL1.
  • Sources of CAND1 and UBE2M not clear.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBE2M CAND1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
CAND1 UBE2M
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High8.815BioGRID
2539798
CAND1 UBE2M
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID