BAIT

KIAA0226

RUBICON, SCAR15, hCG_22771
KIAA0226
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

MTOR

FRAP, FRAP1, FRAP2, RAFT1, RAPT1
mechanistic target of rapamycin (serine/threonine kinase)
GO Process (30)
GO Function (9)
GO Component (11)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

mTORC1 phosphorylates UVRAG to negatively regulate autophagosome and endosome maturation.

Kim YM, Jung CH, Seo M, Kim EK, Park JM, Bae SS, Kim DH

mTORC1 plays a key role in autophagy as a negative regulator. The currently known targets of mTORC1 in the autophagy pathway mainly function at early stages of autophagosome formation. Here, we identify that mTORC1 inhibits later stages of autophagy by phosphorylating UVRAG. Under nutrient-enriched conditions, mTORC1 binds and phosphorylates UVRAG. The phosphorylation positively regulates the association of UVRAG with RUBICON, ... [more]

Mol. Cell Jan. 22, 2015; 57(2);207-18 [Pubmed: 25533187]

Throughput

  • Low Throughput

Curated By

  • BioGRID