BAIT

CDCA3

GRCC8, TOME-1
cell division cycle associated 3
UPS Project
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

WEE1

WEE1A, WEE1hu
WEE1 G2 checkpoint kinase
Cell Cycle - Mitotic Spindle Assembly Project
GO Process (5)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC.

Ayad NG, Rankin S, Murakami M, Jebanathirajah J, Gygi S, Kirschner MW

Entry into mitosis requires the activation of cdk1/cyclin B, while mitotic exit is achieved when the same kinase activity decreases, as cyclin B is degraded. Cyclin B proteolysis is mediated by the anaphase promoting complex, or APC, an E3 ligase that is active at anaphase in mitosis through G1. We have identified a G1 substrate of the APC that we ... [more]

Cell Apr. 04, 2003; 113(1);101-13 [Pubmed: 12679038]

Throughput

  • Low Throughput

Curated By

  • BioGRID