BAIT

EOL2

ETO1-like 2, MZN1.1, AT5G58550
protein ETO1-like 2
GO Process (1)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

ACS5

1-AMINO-1-CYCLOPROPANECARBOXYLATE SYNTHASE, ACC synthase 5, ATACS5, CIN5, CYTOKININ-INSENSITIVE 5, ETHYLENE OVERPRODUCER 2, ETO2, MPA24.15, MPA24_15, AT5G65800
1-aminocyclopropane-1-carboxylate synthase 5
GO Process (4)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Regulation of ethylene gas biosynthesis by the Arabidopsis ETO1 protein.

Wang KL, Yoshida H, Lurin C, Ecker JR

Ethylene gas is used as a hormone by plants, in which it acts as a critical growth regulator. Its synthesis is also rapidly evoked in response to a variety of biotic and abiotic stresses. The Arabidopsis ethylene-overproducer mutants eto2 and eto3 have previously been identified as having mutations in two genes, ACS5 and ACS9, respectively; these encode isozymes of 1-aminocyclopropane-1-carboxylic ... [more]

Nature Apr. 29, 2004; 428(6986);945-50 [Pubmed: 15118728]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EOL2 ACS5
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID