Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Inter- and intra-molecular interactions of Arabidopsis thaliana DELLA protein RGL1.

Sheerin DJ, Buchanan J, Kirk C, Harvey D, Sun X, Spagnuolo J, Li S, Liu T, Woods VA, Foster T, Jones WT, Rakonjac J

The phytohormone gibberellin and the DELLA proteins act together to control key aspects of plant development. Gibberellin induces degradation of DELLA proteins by recruitment of an F-box protein, using a molecular switch: a gibberellin-bound nuclear receptor interacts with the N-terminal domain of DELLA proteins, and this event primes the DELLA C-terminal domain for interaction with the F-box protein. However, the ... [more]

Unknown Feb. 15, 2011; 0(0); [Pubmed: 21323638]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RGL1 GID1A	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Altmann M (2020)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GID1A

Gene Ontology Biological Process

Gene Ontology Molecular Function

gibberellin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

RGL1