BAIT
CAND1
TIP120, TIP120A
cullin-associated and neddylation-dissociated 1
GO Process (4)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CCNB1
CCNB
cyclin B1
GO Process (13)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- mitotic cell cycle [TAS]
- mitotic metaphase plate congression [IMP]
- mitotic nuclear envelope disassembly [TAS]
- mitotic spindle stabilization [IMP]
- positive regulation of attachment of spindle microtubules to kinetochore [IMP]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- regulation of cell cycle [TAS]
- regulation of mitotic cell cycle spindle assembly checkpoint [IMP]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.
Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]
Cell Dec. 10, 2010; 143(6);951-65 [Pubmed: 21145461]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293t cell (BTO:0002181)
Additional Notes
- All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
- TAP-tagged CAND1
- exogenous expression of bait
Curated By
- BioGRID