PTGIR
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger [TAS]
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IMP]
- blood coagulation [TAS]
- cell-cell signaling [TAS]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISS]
- negative regulation of smooth muscle cell proliferation [IDA]
- positive regulation of GTPase activity [TAS]
- positive regulation of cAMP biosynthetic process [IMP]
- positive regulation of cAMP-mediated signaling [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PDE6D
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Internalization and recycling of the human prostacyclin receptor is modulated through its isoprenylation-dependent interaction with the delta subunit of cGMP phosphodiesterase 6.
Prostacyclin, the major cyclooxygenase-derived product of arachidonic acid formed in the vasculature, mediates its potent anti-thrombotic and anti-proliferative effects through its G protein-coupled receptor (GPCR) termed the IP. Unlike many GPCRs, agonist-induced internalization of the IP occurs in an arrestin/GPCR kinase-independent manner. However, deletion of the IP COOH-terminal region prevented internalization suggesting that protein interactions at this region are involved ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PTGIR PDE6D | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 901907 |
Curated By
- BioGRID