BAIT

PDR3

AMY2, TPE2, drug-responsive transcription factor PDR3, L000002985, L000001363, YBL005W
Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate; PDR3 has a paralog, PDR1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

PDR5

LEM1, STS1, YDR1, ATP-binding cassette multidrug transporter PDR5, L000001365, L000002136, L000002504, YOR153W
Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Ribosome quality control is a central protection mechanism for yeast exposed to deoxynivalenol and trichothecin.

Kugler KG, Jandric Z, Beyer R, Klopf E, Glaser W, Lemmens M, Shams M, Mayer K, Adam G, Schueller C

The trichothecene mycotoxins deoxynivalenol (DON) and trichothecin (TTC) are inhibitors of eukaryotic protein synthesis. Their effect on cellular homeostasis is poorly understood. We report a systematic functional investigation of the effect of DON and TTC on the yeast Saccharomyces cerevisiae using genetic array, network and microarray analysis. To focus the genetic analysis on intracellular consequences of toxin action we eliminated ... [more]

BMC Genomics Jun. 02, 2016; 17(1);417 [Pubmed: 27245696]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)
  • resistance to chemicals (APO:0000087)

Additional Notes

  • Figure 1a
  • PDR5 overexpression rescues growth of pdr1 pdr3 double mutant on deoxynivalenol and trichothecin
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PDR3 PDR5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
PDR5 PDR3
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
154966
PDR3 PDR5
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
2208747

Curated By

  • BioGRID