BAIT

UGGT-1

CELE_F48E3.3, F48E3.3
uggt-1 encodes one of two C. elegans homologs of UDP-Glc:glycoprotein glucosyltransferase (UGGT), an endoplasmic reticulum enzyme involved in quality control of glycoprotein folding; expression of uggt-1 in S. pombe cells lacking functional UGGT activity indicates that UGGT-1 displays UDP-Glc:glycoprotein glucosyltransferase activity; loss of uggt-1 activity via RNAi results in larval developmental delay and a shortened lifespan; uggt-1 is expressed throughout larval development, with peak levels seen at the L2/L3 stages, and uggt-1 expression is upregulated in an ire-1-dependent manner in response to endoplasmic reticulum stress; a uggt-1::gfp promoter fusion is expressed in the nervous system, including the amphid and phasmid neurons, the nerve ring, and the dorsal and ventral nerve cords.
Caenorhabditis elegans
PREY

UDA-1

CELE_K08H10.4, K08H10.4
Uridine DiphosphAtase
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Caenorhabditis elegans

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

EPIC: software toolkit for elution profile-based inference of protein complexes.

Hu LZ, Goebels F, Tan JH, Wolf E, Kuzmanov U, Wan C, Phanse S, Xu C, Schertzberg M, Fraser AG, Bader GD, Emili A

Protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We and others previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to precision mass spectrometry analysis (chromatographic fractionation-mass spectrometry, CF-MS), but the resulting data are challenging to process and interpret. Here, we describe EPIC ... [more]

Nat. Methods Jul. 15, 2019; (); [Pubmed: 31308550]

Quantitative Score

  • 0.74 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • High confidence protein interactions have an EPIC PPI score >= 0.5

Curated By

  • BioGRID