BAIT
CA6
CA-VI, GUSTIN, RP3-477M7.7
carbonic anhydrase VI
GO Process (3)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PCSK9
FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PSEC0052
proprotein convertase subtilisin/kexin type 9
GO Process (17)
GO Function (11)
GO Component (11)
Gene Ontology Biological Process
- cellular response to insulin stimulus [ISS]
- cellular response to starvation [ISS]
- cholesterol homeostasis [IMP]
- kidney development [ISS]
- liver development [ISS]
- low-density lipoprotein particle receptor catabolic process [IDA]
- lysosomal transport [IDA]
- negative regulation of low-density lipoprotein particle clearance [IDA]
- negative regulation of receptor recycling [IDA]
- neurogenesis [ISS]
- neuron differentiation [ISS]
- positive regulation of neuron apoptotic process [IMP]
- positive regulation of receptor internalization [IDA]
- protein autoprocessing [IDA]
- proteolysis [IBA]
- regulation of neuron apoptotic process [ISS]
- regulation of receptor activity [IDA]
Gene Ontology Molecular Function- apolipoprotein binding [ISS]
- apolipoprotein receptor binding [IDA]
- low-density lipoprotein particle binding [ISS]
- low-density lipoprotein particle receptor binding [IDA, IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein self-association [IDA]
- serine-type endopeptidase activity [IDA]
- sodium channel inhibitor activity [IDA]
- very-low-density lipoprotein particle binding [ISS]
- very-low-density lipoprotein particle receptor binding [IDA]
- apolipoprotein binding [ISS]
- apolipoprotein receptor binding [IDA]
- low-density lipoprotein particle binding [ISS]
- low-density lipoprotein particle receptor binding [IDA, IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein self-association [IDA]
- serine-type endopeptidase activity [IDA]
- sodium channel inhibitor activity [IDA]
- very-low-density lipoprotein particle binding [ISS]
- very-low-density lipoprotein particle receptor binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.905254555 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.905254555, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID