BAIT

N

nuc, nucleocapsid, SARS-CoV2 N, N protein, ncap, SARS-CoV-2 N, Nucleoprotein, NCAP_SARS2, PRO_0000449656, GU280_gp10
Nucleocapsid phosphoprotein; Nucleoprotein
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

ANXA1

ANX1, LPC1, RP11-71A24.1
annexin A1
Arachidonic Acid Pathway Project
Human Papilloma Virus (HPV) Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets.

Somasekharan SP, Gleave M

The current work investigated SARS-CoV-2 Nucleocapsid (NCAP or N protein) interactors in A549 human lung cancer cells using a SILAC-based mass spectrometry approach. NCAP interactors included proteins of the stress granule (SG) machinery and immunoregulators. NCAP showed specific interaction with the SG proteins G3BP1, G3BP2, YTHDF3, USP10 and PKR, and translocated to SGs following oxidative stress and heat shock. Treatment ... [more]

FEBS Lett Dec. 01, 2020; 595(23);2872-2896 [Pubmed: 34780058]

Throughput

  • High Throughput

Additional Notes

  • Data were organized into four categories of SARS-CoV-2 N-associated proteins after arsenite stress: interactions that were stress-enhanced, stress-dependent, stress-independent and non-stress-specific.
  • Non-stress-specific interaction (Table 1)

Curated By

  • BioGRID