Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A novel cardioprotective p38-MAPK/mTOR pathway.

Hernandez G, Lal H, Fidalgo M, Guerrero A, Zalvide J, Force T, Pombo CM

Despite intensive study, the mechanisms regulating activation of mTOR and the consequences of that activation in the ischemic heart remain unclear. This is particularly true for the setting of ischemia/reperfusion (I/R) injury. In a mouse model of I/R injury, we observed robust mTOR activation, and its inhibition by rapamycin increased injury. Consistent with the in-vivo findings, mTOR activation was also ... [more]

Exp. Cell Res. Dec. 10, 2011; 317(20);2938-49 [Pubmed: 22001647]

Throughput

  • Low Throughput

Additional Notes

  • unclear which 14-3-3 protein used as bait

Curated By

  • BioGRID