BAIT

ESP1

L000000584, YGR098C
Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

CDC20

PAC5, ubiquitin-protein transferase activating protein CDC20, L000000259, YGL116W
Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
UPS Project
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage.

Tinker-Kulberg RL, Morgan DO

The separation of sister chromatids in anaphase is followed by spindle disassembly and cytokinesis. These events are governed by the anaphase-promoting complex (APC), which triggers the ubiquitin-dependent proteolysis of key regulatory proteins: anaphase requires the destruction of the anaphase inhibitor Pds1, whereas mitotic exit requires the destruction of mitotic cyclins and the inactivation of Cdk1. We find that Pds1 is ... [more]

Genes Dev. Aug. 01, 1999; 13(15);1936-49 [Pubmed: 10444592]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cell cycle progression in m phase (APO:0000258)

Additional Notes

  • Cyclin destruction promoted by over-expression of Esp1 was suppressed by mutation of CDC20

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDC20 ESP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.6375BioGRID
1933184

Curated By

  • BioGRID