BAIT

SNF1

CAT1, CCR1, GLC2, HAF3, PAS14, AMP-activated serine/threonine-protein kinase catalytic subunit SNF1, L000001944, YDR477W
AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ubiquitin ligase
Kinome Project (Sc)
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

RIM101

RIM1, alkaline-responsive transcritional regulator RIM101, L000002818, YHL027W
Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Co-regulated expression of Na+/phosphate Pho89 transporter and Ena1 Na+-ATPase allows their functional coupling under high pH stress.

Serra-Cardona A, Petrezselyova S, Canadell D, Ramos J, Arino J

The yeast Saccharomyces cerevisiae has two main high-affinity inorganic phosphate (Pi) transporters, Pho84 and Pho89, functionally relevant at acidic/neutral pH and alkaline pH, respectively. Upon Pi starvation PHO84 and PHO89 are induced by activation of the PHO regulon, by binding of the Pho4 transcription factor to specific promoter sequences. We show that PHO89 and PHO84 are induced by alkalinization of ... [more]

Mol. Cell. Biol. Sep. 29, 2014; 0(0); [Pubmed: 25266663]

Throughput

  • Low Throughput

Ontology Terms

  • protein activity (APO:0000022)

Additional Notes

  • Figure 4
  • snf1 rim101 double mutant has decreased induction of PHO89 via LacZ reporter

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RIM101 SNF1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-4.9464BioGRID
542194

Curated By

  • BioGRID