BAIT

SIW14

OCA3, putative tyrosine protein phosphatase SIW14, L000004216, YNL032W
Tyrosine phosphatase involved in actin organization and endocytosis; localized to the cytoplasm
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

PPH21

phosphoprotein phosphatase 2A catalytic subunit PPH21, L000001472, YDL134C
Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The protein phosphatase Siw14 controls caffeine-induced nuclear localization and phosphorylation of Gln3 via the type 2A protein phosphatases Pph21 and Pph22 in Saccharomyces cerevisiae.

Numamoto M, Sasano Y, Hirasaki M, Sugiyama M, Maekawa H, Harashima S

The Saccharomyces cerevisiae Siw14, a tyrosine phosphatase involved in the response to caffeine, participates in regulation of the phosphorylation and intracellular localization of Gln3, a GATA transcriptional activator of nitrogen catabolite repression-sensitive genes. In Δsiw14 cells, the phosphorylation level of Gln3 is decreased and the nuclear localization of Gln3 is stimulated by caffeine. However, the mechanism by which Siw14 controls ... [more]

J. Biochem. Oct. 13, 2014; 0(0); [Pubmed: 25313402]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide modification (APO:0000131)
  • phenotype: protein/peptide distribution (APO:0000209)

Additional Notes

  • Figure 4
  • Figure 5
  • PPH21 PPH22 double deletion inhibits Gln3 dephosphorylation in siw14 mutant
  • PPH21 PPH22 double deletion suppresses increased Gln3 nuclear localization in siw14 mutant
  • PPH21 deletion inhibits Gln3 dephosphorylation in siw14 mutant
  • PPH21 deletion suppresses increased Gln3 nuclear localization in siw14 mutant
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PPH21 SIW14
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3401BioGRID
363347
PPH21 SIW14
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4275BioGRID
2090675

Curated By

  • BioGRID