GPA2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GPA1
Gene Ontology Biological Process
- adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [IMP]
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IBA]
- heterotrimeric G-protein complex cycle [IMP]
- inositol lipid-mediated signaling [IMP]
- karyogamy involved in conjugation with cellular fusion [IMP]
- nuclear migration involved in conjugation with cellular fusion [IMP]
- pheromone-dependent signal transduction involved in conjugation with cellular fusion [IMP]
- regulation of MAPK export from nucleus [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.
The recent abundance of genome sequence data has brought an urgent need for systematic proteomics to decipher the encoded protein networks that dictate cellular function. To date, generation of large-scale protein-protein interaction maps has relied on the yeast two-hybrid system, which detects binary interactions through activation of reporter gene expression. With the advent of ultrasensitive mass spectrometric protein identification methods, ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
GPA2 GPA1 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | - | |
GPA2 GPA1 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 158107 |
Curated By
- BioGRID