BAIT

RPT3

YNT1, YTA2, proteasome regulatory particle base subunit RPT3, L000002556, L000002537, YDR394W

ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B

UPS Project

GO Process (3)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IMP]

proteasome regulatory particle assembly [IMP]

ubiquitin-dependent protein catabolic process [IMP]

Gene Ontology Molecular Function

ATPase activity [ISS]

Gene Ontology Cellular Component

proteasome regulatory particle, base subcomplex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPN11

MPR1, proteasome regulatory particle lid subunit RPN11, L000002976, L000002965, YFR004W

Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

UPS Project

GO Process (4)

GO Function (2)

GO Component (5)

Gene Ontology Biological Process

mitochondrial fission [IMP]

peroxisome fission [IMP]

proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]

protein deubiquitination [IGI, IMP]

Gene Ontology Molecular Function

metallopeptidase activity [ISS]
ubiquitin-specific protease activity [IMP]

Gene Ontology Cellular Component

cytosol [IDA]

mitochondrion [IDA]

nucleus [IDA]

proteasome regulatory particle, lid subcomplex [IDA]

proteasome storage granule [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.

Ho Y, Gruhler A, Heilbut A, Bader GD, Moore L, Adams SL, Millar A, Taylor P, Bennett K, Boutilier K, Yang L, Wolting C, Donaldson I, Schandorff S, Shewnarane J, Vo M, Taggart J, Goudreault M, Muskat B, Alfarano C, Dewar D, Lin Z, Michalickova K, Willems AR, Sassi H, Nielsen PA, Rasmussen KJ, Andersen JR, Johansen LE, Hansen LH, Jespersen H, Podtelejnikov A, Nielsen E, Crawford J, Poulsen V, Sorensen BD, Matthiesen J, Hendrickson RC, Gleeson F, Pawson T, Moran MF, Durocher D, Mann M, Hogue CW, Figeys D, Tyers M

The recent abundance of genome sequence data has brought an urgent need for systematic proteomics to decipher the encoded protein networks that dictate cellular function. To date, generation of large-scale protein-protein interaction maps has relied on the yeast two-hybrid system, which detects binary interactions through activation of reporter gene expression. With the advent of ultrasensitive mass spectrometric protein identification methods, ... [more]

Nature Jan. 10, 2002; 415(6868);180-3 [Pubmed: 11805837]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Babu M (2012)	High	-	BioGRID	694006
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gomez TA (2011)	Low	-	BioGRID	-
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Guerrero C (2006)	High	-	BioGRID	-
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kimura Y (2013)	High	-	BioGRID	1035809
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Matyskiela ME (2013)	Low	-	BioGRID	-
RPT3 RPN11	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	6	BioGRID	3612807
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Panasenko OO (2011)	High	-	BioGRID	-
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sakata E (2011)	Low	-	BioGRID	568488
RPN11 RPT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Yu Z (2015)	Low	-	BioGRID	-
RPN11 RPT3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Beck F (2012)	Low	-	BioGRID	938915
RPN11 RPT3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cresti JR (2021)	Low	-	BioGRID	-
RPN11 RPT3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cundiff MD (2019)	Low	-	BioGRID	-
RPN11 RPT3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Leggett DS (2005)	Low	-	BioGRID	449947
RPN11 RPT3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Reichard EL (2016)	Low	-	BioGRID	-
RPN11 RPT3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Saeki Y (2005)	Low	-	BioGRID	-
RPN11 RPT3	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Luan B (2016)	Low	-	BioGRID	-
RPN11 RPT3	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Sakata E (2012)	Low	-	BioGRID	1034573
RPT3 RPN11	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5407	BioGRID	1927706
RPN11 RPT3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3397	BioGRID	1931641
RPN11 RPT3	Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.	Breslow DK (2008)	High	0.028	BioGRID	443067
RPT3 RPN11	Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.	Breslow DK (2008)	High	0.028	BioGRID	443068
RPN11 RPT3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ding Z (2017)	Low	-	BioGRID	-
RPN11 RPT3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lander GC (2012)	Low	-	BioGRID	-
RPT3 RPN11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Yu Z (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RPT3

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [ISS]

Gene Ontology Cellular Component

RPN11

Gene Ontology Biological Process

Gene Ontology Molecular Function

metallopeptidase activity [ISS]ubiquitin-specific protease activity [IMP]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.

Throughput

Related interactions

Curated By

metallopeptidase activity [ISS]
ubiquitin-specific protease activity [IMP]