BAIT

BMH1

APR6, 14-3-3 family protein BMH1, L000000185, YER177W
14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

SRB8

GIG1, MED12, NUT6, SSN5, YCR080W, L000003007, YCR081W
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

14-3-3 functions as a comodulator of transcription by inhibiting coactivator functions.

Parua PK, Dombek KM, Young ET

In eukaryotes combinatorial activation of transcription is an important component of gene regulation. In the budding yeast Saccharomyces cerevisiae, Adr1-Cat8 and Adr1-Oaf1/Pip2 are pairs of activators that act together to regulate two diverse sets of genes. Transcription activation of both sets is regulated positively by the yeast AMPK homologue, Snf1 in response to low glucose or the presence of a ... [more]

J. Biol. Chem. Oct. 29, 2014; 0(0); [Pubmed: 25355315]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: rna accumulation (APO:0000224)

Additional Notes

  • Figure 11C
  • bmh1-ts srb8 double mutant has increased ADH2 expression

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BMH1 SRB8
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.531BioGRID
220630

Curated By

  • BioGRID