BAIT

ASI3

putative ubiquitin-protein ligase ASI3, YNL008C
Subunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids; ASI3 has a paralog, ASI1, that arose from the whole genome duplication
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

ERG11

CYP51, sterol 14-demethylase, L000000577, YHR007C
Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Quality control of inner nuclear membrane proteins by the Asi complex.

Foresti O, Rodriguez-Vaello V, Funaya C, Carvalho P

Misfolded proteins in the endoplasmic reticulum (ER) are eliminated by a quality-control system called ER-associated protein degradation (ERAD). However, how misfolded proteins in the inner nuclear membrane (INM), a specialized ER subdomain, are degraded is not known. Here, a quantitative proteomics approach revealed an ERAD branch required for INM protein quality control in yeast. This branch involved the integral membrane ... [more]

Science Sep. 18, 2014; 0(0); [Pubmed: 25236469]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ASI3 ERG11
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1222BioGRID
2063801

Curated By

  • BioGRID