BAIT

MTUS2

CAZIP, ICIS, KIAA0774, TIP150
microtubule associated tumor suppressor candidate 2
GO Process (0)
GO Function (3)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

KIF2C

CT139, KNSL6, MCAK, RP11-269F19.1
kinesin family member 2C
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of a dynamic interaction between two microtubule-binding proteins, EB1 and TIP150, by the mitotic p300/CBP-associated factor (PCAF) orchestrates kinetochore microtubule plasticity and chromosome stability during mitosis.

Ward T, Wang M, Liu X, Wang Z, Xia P, Chu Y, Wang X, Liu L, Jiang K, Yu H, Yan M, Wang J, Hill DL, Huang Y, Zhu T, Yao X

The microtubule cytoskeleton network orchestrates cellular dynamics and chromosome stability in mitosis. Although tubulin acetylation is essential for cellular plasticity, it has remained elusive how kinetochore microtubule plus-end dynamics are regulated by p300/CBP-associated factor (PCAF) acetylation in mitosis. Here, we demonstrate that the plus-end tracking protein, TIP150, regulates dynamic kinetochore-microtubule attachments by promoting the stability of spindle microtubule plus-ends. Suppression ... [more]

J. Biol. Chem. May. 31, 2013; 288(22);15771-85 [Pubmed: 23595990]

Throughput

  • Low Throughput

Additional Notes

  • Figure 5

Curated By

  • BioGRID