BAIT
PTPN11
Shp2
protein tyrosine phosphatase, non-receptor type 11
GO Process (41)
GO Function (12)
GO Component (5)
Gene Ontology Biological Process
- DNA damage checkpoint [ISO]
- ERBB signaling pathway [ISO]
- abortive mitotic cell cycle [ISO]
- activation of MAPK activity [ISO]
- atrioventricular canal development [ISO]
- axonogenesis [ISO]
- brain development [ISO]
- dephosphorylation [ISO]
- ephrin receptor signaling pathway [ISO, ISS]
- epidermal growth factor receptor signaling pathway [ISO]
- face morphogenesis [ISO]
- genitalia development [ISO]
- glucose homeostasis [ISO]
- heart development [ISO]
- hormone metabolic process [ISO]
- hormone-mediated signaling pathway [ISO]
- inner ear development [ISO]
- integrin-mediated signaling pathway [ISO]
- lipid metabolic process [ISO]
- megakaryocyte development [ISO]
- multicellular organismal reproductive process [ISO]
- negative regulation of cell adhesion mediated by integrin [ISO]
- negative regulation of cortisol secretion [ISO]
- negative regulation of growth hormone secretion [ISO]
- negative regulation of hormone secretion [ISO]
- negative regulation of insulin secretion [ISO]
- neurotrophin TRK receptor signaling pathway [ISO]
- organ growth [ISO]
- peptidyl-tyrosine dephosphorylation [ISO, ISS]
- platelet formation [ISO]
- platelet-derived growth factor receptor signaling pathway [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of hormone secretion [ISO]
- positive regulation of mitotic cell cycle [ISO]
- positive regulation of signal transduction [ISO]
- protein dephosphorylation [IDA]
- regulation of cell adhesion mediated by integrin [ISO, ISS]
- regulation of multicellular organism growth [ISO]
- regulation of protein export from nucleus [ISO]
- triglyceride metabolic process [ISO]
Gene Ontology Molecular Function- D1 dopamine receptor binding [IPI]
- SH3/SH2 adaptor activity [ISO]
- insulin receptor binding [IDA, ISO]
- insulin receptor substrate binding [IPI]
- non-membrane spanning protein tyrosine phosphatase activity [ISO, ISS]
- peptide hormone receptor binding [ISO]
- phospholipase binding [IDA]
- phosphoprotein phosphatase activity [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein tyrosine phosphatase activity [IDA, ISO]
- receptor tyrosine kinase binding [ISO]
- D1 dopamine receptor binding [IPI]
- SH3/SH2 adaptor activity [ISO]
- insulin receptor binding [IDA, ISO]
- insulin receptor substrate binding [IPI]
- non-membrane spanning protein tyrosine phosphatase activity [ISO, ISS]
- peptide hormone receptor binding [ISO]
- phospholipase binding [IDA]
- phosphoprotein phosphatase activity [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein tyrosine phosphatase activity [IDA, ISO]
- receptor tyrosine kinase binding [ISO]
Rattus norvegicus
PREY
IRS1
IRS1IRM
insulin receptor substrate 1
GO Process (28)
GO Function (10)
GO Component (9)
Gene Ontology Biological Process
- cellular response to insulin stimulus [ISO]
- insulin receptor signaling pathway [IDA, ISO]
- insulin secretion [ISO]
- insulin-like growth factor receptor signaling pathway [ISO, ISS]
- lipid catabolic process [ISO]
- mammary gland development [ISO]
- negative regulation of insulin receptor signaling pathway [IMP]
- negative regulation of insulin secretion [IMP, ISO]
- negative regulation of somatostatin secretion [IMP]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of glucagon secretion [IMP]
- positive regulation of glucose import [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of insulin receptor signaling pathway [IMP]
- positive regulation of mesenchymal cell proliferation [ISO]
- positive regulation of phosphatidylinositol 3-kinase activity [IDA, ISO]
- positive regulation of phosphorylation [IDA]
- protein heterooligomerization [IDA]
- protein kinase B signaling [ISO]
- protein localization to nucleus [ISO]
- regulation of gene expression [ISO]
- response to caffeine [IDA]
- response to insulin [ISO]
- response to peptide hormone [IDA]
Gene Ontology Molecular Function- SH2 domain binding [IPI]
- insulin receptor binding [IMP, ISO, ISS]
- insulin-like growth factor receptor binding [IPI, ISO, ISS]
- phosphatidylinositol 3-kinase binding [IDA, IMP, IPI, ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase C binding [IPI]
- protein kinase binding [IPI, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [IMP]
- SH2 domain binding [IPI]
- insulin receptor binding [IMP, ISO, ISS]
- insulin-like growth factor receptor binding [IPI, ISO, ISS]
- phosphatidylinositol 3-kinase binding [IDA, IMP, IPI, ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase C binding [IPI]
- protein kinase binding [IPI, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [IMP]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Early signaling events triggered by peroxovanadium [bpV(phen)] are insulin receptor kinase (IRK)-dependent: specificity of inhibition of IRK-associated protein tyrosine phosphatase(s) by bpV(phen).
Peroxovanadiums (pVs) are potent protein tyrosine phosphatase (PTP) inhibitors with insulin-mimetic properties in vivo and in vitro. We have established the existence of an insulin receptor kinase (IRK)-associated PTP whose inhibition by pVs correlates closely with IRK tyrosine phosphorylation, activation, and downstream signaling. pVs have also been shown to activate various tyrosine kinases (TKs) that could participate in activation of ... [more]
Mol. Endocrinol. Dec. 01, 1997; 11(13);1899-910 [Pubmed: 9415395]
Throughput
- Low Throughput
Curated By
- BioGRID