BAIT

RNF7

CKBBP1, ROC2, SAG
ring finger protein 7
UPS Project
Homo sapiens
PREY

UBE2M

UBC-RS2, UBC12, hUbc12
ubiquitin-conjugating enzyme E2M
Cell Cycle - Mitotic Spindle Assembly Project
UPS Project
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.

Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA

Ubiquitin and ubiquitin-like proteins (UBLs) are directed to targets by cascades of E1, E2, and E3 enzymes. The largest ubiquitin E3 subclass consists of cullin-RING ligases (CRLs), which contain one each of several cullins (CUL1, -2, -3, -4, or -5) and RING proteins (RBX1 or -2). CRLs are activated by ligation of the UBL NEDD8 to a conserved cullin lysine. ... [more]

Mol. Cell Feb. 27, 2009; 33(4);483-95 [Pubmed: 19250909]

Throughput

  • Low Throughput

Additional Notes

  • E2:UBE2M

Curated By

  • BioGRID