BAIT

VAMP2

Syb-2, Syb2, sybII, RP23-26L6.8
vesicle-associated membrane protein 2
GO Process (15)
GO Function (12)
GO Component (23)
Mus musculus
PREY

NAPB

Brp14, E161, I47, SNARE, b-SNAP, RP23-377E1.3
N-ethylmaleimide sensitive fusion protein attachment protein beta
Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dynamin-1 co-associates with native mouse brain BKCa channels: proteomics analysis of synaptic protein complexes.

Gorini G, Ponomareva O, Shores KS, Person MD, Harris RA, Mayfield RD

In every synapse, a large number of proteins interact with other proteins in order to carry out signaling and transmission in the central nervous system. In this study, we used interaction proteomics to identify novel synaptic protein interactions in mouse cortical membranes under native conditions. Using immunoprecipitation, immunoblotting, and mass spectrometry, we identified a number of novel synaptic protein interactions ... [more]

FEBS Lett. Mar. 05, 2010; 584(5);845-51 [Pubmed: 20114047]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NAPB VAMP2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.9775BioGRID
2676891

Curated By

  • BioGRID