An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin.

Klein UR, Haindl M, Nigg EA, Muller S

The ubiquitin-like SUMO system controls cellular key functions, and several lines of evidence point to a critical role of SUMO for mitotic progression. However, in mammalian cells mitotic substrates of sumoylation and the regulatory components involved are not well defined. Here, we identify Borealin, a component of the chromosomal passenger complex (CPC), as a mitotic target of SUMO. The CPC, ... [more]

Mol. Biol. Cell Jan. 01, 2009; 20(1);410-8 [Pubmed: 18946085]

Throughput

Low Throughput

Additional Notes

E1: Aos1/Uba2
Figure 1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDCA8 UBE2I	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Klein UR (2009)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UBE2I

Gene Ontology Biological Process

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]SUMO transferase activity [IDA]enzyme binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]transcription factor binding [IPI]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

CDCA8