AKT2
Gene Ontology Biological Process
- cellular protein modification process [TAS]
- cellular response to insulin stimulus [IMP]
- fat cell differentiation [TAS]
- insulin receptor signaling pathway [IMP, TAS]
- intracellular protein transmembrane transport [ISS]
- mammary gland epithelial cell differentiation [TAS]
- membrane organization [TAS]
- negative regulation of plasma membrane long-chain fatty acid transport [IMP]
- positive regulation of cell motility [IMP]
- positive regulation of fatty acid beta-oxidation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP]
- positive regulation of protein phosphorylation [ISS]
- positive regulation of protein targeting to membrane [ISS]
- positive regulation of vesicle fusion [ISS]
- regulation of cell cycle arrest [TAS]
- regulation of cell migration [TAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell cortex [ISS]
- cytosol [TAS]
- nucleus [IDA, TAS]
- plasma membrane [ISS, TAS]
- ruffle membrane [ISS]
APPL1
Gene Ontology Biological Process
- apoptotic process [TAS]
- cell proliferation [IDA]
- insulin receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- regulation of apoptotic process [TAS]
- regulation of establishment of protein localization to plasma membrane [IMP]
- regulation of glucose import [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2.
AKT2 is a serine/threonine kinase implicated in human ovarian and pancreatic cancers. AKT2 is activated by a variety of growth factors and insulin via phosphatidylinositol 3-kinase (PI3K). However, its normal cellular role is not well understood. To gain insight into the function of AKT2, we performed yeast two-hybrid system to screen for interacting proteins. Using this technique, we identified a ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AKT2 APPL1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| APPL1 AKT2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 798428 | |
| APPL1 AKT2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID