An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cdh1 regulates osteoblast function through an APC/C-independent modulation of Smurf1.

Wan L, Zou W, Gao D, Inuzuka H, Fukushima H, Berg AH, Drapp R, Shaik S, Hu D, Lester C, Eguren M, Malumbres M, Glimcher LH, Wei W

The APC/Cdh1 E3 ubiquitin ligase plays an essential role in both mitotic exit and G1/S transition by targeting key cell-cycle regulators for destruction. There is mounting evidence indicating that Cdh1 has other functions in addition to cell-cycle regulation. However, it remains unclear whether these additional functions depend on its E3 ligase activity. Here, we report that Cdh1, but not Cdc20, ... [more]

Mol. Cell Dec. 09, 2011; 44(5);721-33 [Pubmed: 22152476]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CDH2

Gene Ontology Biological Process

Gene Ontology Molecular Function

alpha-catenin binding [IPI]beta-catenin binding [TAS]gamma-catenin binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

CCNA2