An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation.

Duda DM, Borg LA, Scott DC, Hunt HW, Hammel M, Schulman BA

Cullin-RING ligases (CRLs) comprise the largest ubiquitin E3 subclass, in which a central cullin subunit links a substrate-binding adaptor with an E2-binding RING. Covalent attachment of the ubiquitin-like protein NEDD8 to a conserved C-terminal domain (ctd) lysine stimulates CRL ubiquitination activity and prevents binding of the inhibitor CAND1. Here we report striking conformational rearrangements in the crystal structure of NEDD8~Cul5(ctd)-Rbx1 ... [more]

Cell Sep. 19, 2008; 134(6);995-1006 [Pubmed: 18805092]

Throughput

Low Throughput

Additional Notes

Figure S2

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBE2M CUL5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Scott DC (2017)	High	-	BioGRID	-
CUL5 UBE2M	Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.	Han K (2017)	High	11.319	BioGRID	2539821

Curated By

BioGRID

Interaction Summary

UBE2M

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8 transferase activity [IDA]protein binding [IPI]ribosomal S6-glutamic acid ligase activity [IDA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [IMP, TAS]

Gene Ontology Cellular Component

CUL5

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium channel activity [TAS]protein binding [IPI]receptor activity [TAS]ubiquitin protein ligase binding [IDA]