BAIT
BCL2
Bcl-2
B-cell CLL/lymphoma 2
GO Process (156)
GO Function (14)
GO Component (14)
Gene Ontology Biological Process
- B cell differentiation [ISO]
- B cell homeostasis [ISO]
- B cell lineage commitment [ISO]
- B cell proliferation [ISO]
- B cell receptor signaling pathway [ISO]
- CD8-positive, alpha-beta T cell lineage commitment [ISO]
- T cell differentiation [ISO]
- T cell differentiation in thymus [ISO]
- T cell homeostasis [ISO]
- T cell lineage commitment [ISO]
- actin filament organization [ISO]
- aging [IEP]
- apoptotic mitochondrial changes [ISO]
- apoptotic process [IEP, ISO, TAS]
- axon regeneration [ISO]
- axonogenesis [ISO]
- behavioral fear response [ISO]
- brain development [IEP]
- branching involved in ureteric bud morphogenesis [ISO]
- cell aging [ISO]
- cell growth [ISO]
- cell morphogenesis [ISO]
- cell proliferation [ISO]
- cellular calcium ion homeostasis [ISO]
- cellular response to DNA damage stimulus [ISO]
- cellular response to glucose starvation [ISO]
- cellular response to hypoxia [ISO]
- cellular response to organic substance [ISO]
- cochlear nucleus development [ISO]
- defense response to virus [ISO]
- developmental growth [ISO]
- developmental pigmentation [ISO]
- digestive tract morphogenesis [ISO]
- ear development [ISO]
- endoplasmic reticulum calcium ion homeostasis [ISO]
- extrinsic apoptotic signaling pathway in absence of ligand [IBA, ISO]
- extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- focal adhesion assembly [ISO]
- gland morphogenesis [ISO]
- glial cell apoptotic process [IEP]
- glomerulus development [ISO]
- growth [ISO]
- hair follicle morphogenesis [ISO]
- hemopoiesis [ISO]
- homeostasis of number of cells within a tissue [ISO]
- immune system development [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage [IBA]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO]
- intrinsic apoptotic signaling pathway in response to oxidative stress [ISO]
- kidney development [ISO]
- leukocyte homeostasis [ISO]
- lymphocyte homeostasis [ISO]
- lymphoid progenitor cell differentiation [ISO]
- male gonad development [ISO]
- melanin metabolic process [ISO]
- melanocyte differentiation [ISO]
- mesenchymal cell development [ISO]
- metanephros development [ISO]
- negative regulation of G1/S transition of mitotic cell cycle [ISO]
- negative regulation of anoikis [ISO]
- negative regulation of apoptotic process [IDA, IMP, ISO]
- negative regulation of apoptotic signaling pathway [ISO]
- negative regulation of calcium ion transport into cytosol [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell migration [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of cellular pH reduction [ISO]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISO]
- negative regulation of intrinsic apoptotic signaling pathway [IBA, ISO]
- negative regulation of mitotic cell cycle [ISO]
- negative regulation of myeloid cell apoptotic process [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of ossification [ISO]
- negative regulation of osteoblast proliferation [ISO]
- negative regulation of retinal cell programmed cell death [ISO]
- neuron apoptotic process [ISO]
- oligodendrocyte differentiation [IEP]
- oocyte development [ISO]
- organ growth [ISO]
- organ morphogenesis [ISO]
- ossification [ISO]
- ovarian follicle development [IEP, ISO]
- peptidyl-serine phosphorylation [ISO]
- peptidyl-threonine phosphorylation [ISO]
- pigment granule organization [ISO]
- pigmentation [ISO]
- positive regulation of B cell proliferation [ISO]
- positive regulation of catalytic activity [ISO]
- positive regulation of cell growth [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of developmental pigmentation [ISO]
- positive regulation of melanocyte differentiation [ISO]
- positive regulation of multicellular organism growth [ISO]
- positive regulation of neuron maturation [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of skeletal muscle fiber development [ISO]
- positive regulation of smooth muscle cell migration [ISO]
- post-embryonic development [ISO]
- protein dephosphorylation [ISO]
- protein polyubiquitination [ISO]
- reactive oxygen species metabolic process [ISO]
- regulation of apoptotic process [ISO]
- regulation of calcium ion transport [ISO]
- regulation of catalytic activity [ISO]
- regulation of cell cycle [ISO]
- regulation of cell-matrix adhesion [ISO]
- regulation of developmental pigmentation [ISO]
- regulation of gene expression [ISO]
- regulation of glycoprotein biosynthetic process [ISO]
- regulation of mitochondrial membrane permeability [ISO, ISS]
- regulation of mitochondrial membrane potential [ISO, ISS]
- regulation of nitrogen utilization [ISO]
- regulation of programmed cell death [ISO]
- regulation of protein heterodimerization activity [ISO]
- regulation of protein homodimerization activity [ISO]
- regulation of protein localization [ISO]
- regulation of protein stability [ISO]
- regulation of transmembrane transporter activity [ISO]
- regulation of viral genome replication [ISO]
- release of cytochrome c from mitochondria [ISO, ISS]
- renal system process [ISO]
- response to L-ascorbic acid [IEP]
- response to UV-B [ISO]
- response to aluminum ion [IEP]
- response to cAMP [IEP]
- response to caffeine [IEP]
- response to copper ion [IEP]
- response to corticosterone [IEP]
- response to cytokine [IEP, ISO]
- response to drug [IEP, ISO]
- response to electrical stimulus [IEP]
- response to estrogen [IEP]
- response to ethanol [IEP]
- response to folic acid [IEP]
- response to gamma radiation [ISO]
- response to glucocorticoid [ISO]
- response to heat [IEP]
- response to hydrogen peroxide [ISO]
- response to hypoxia [IEP]
- response to inorganic substance [IEP]
- response to insulin [IEP]
- response to iron ion [ISO]
- response to ischemia [ISO]
- response to nicotine [IEP, ISO]
- response to nutrient [IEP]
- response to organic cyclic compound [IEP]
- response to organic substance [IEP]
- response to oxidative stress [ISO]
- response to peptide hormone [IEP]
- response to steroid hormone [ISO]
- response to toxic substance [ISO]
- single organismal cell-cell adhesion [ISO]
- spleen development [ISO]
- thymus development [ISO]
- transmembrane transport [ISO]
- ureteric bud development [ISO]
Gene Ontology Molecular Function- BH domain binding [IPI]
- BH3 domain binding [ISO]
- channel activity [ISO]
- channel inhibitor activity [ISO]
- identical protein binding [ISO]
- protease binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IBA, ISO]
- protein phosphatase 2A binding [ISO]
- protein phosphatase binding [ISO]
- sequence-specific DNA binding [ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- BH domain binding [IPI]
- BH3 domain binding [ISO]
- channel activity [ISO]
- channel inhibitor activity [ISO]
- identical protein binding [ISO]
- protease binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IBA, ISO]
- protein phosphatase 2A binding [ISO]
- protein phosphatase binding [ISO]
- sequence-specific DNA binding [ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- cell [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- intracellular [ISO]
- membrane [ISO]
- mitochondrial membrane [ISO]
- mitochondrial outer membrane [IBA, ISO]
- mitochondrion [IDA, ISO]
- myelin sheath [ISO]
- nuclear membrane [ISO]
- nucleus [ISO]
- pore complex [ISO]
Rattus norvegicus
PREY
BAX
Bcl2-associated X protein
GO Process (112)
GO Function (11)
GO Component (17)
Gene Ontology Biological Process
- B cell apoptotic process [ISO, ISS]
- B cell homeostasis [ISO]
- B cell homeostatic proliferation [ISO]
- B cell negative selection [ISO]
- B cell receptor apoptotic signaling pathway [ISO]
- Sertoli cell proliferation [ISO]
- T cell homeostatic proliferation [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO, ISS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c [ISO, ISS, TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [ISO]
- apoptotic mitochondrial changes [ISO, ISS]
- apoptotic process [IEP, IMP, ISO]
- apoptotic process involved in embryonic digit morphogenesis [ISO]
- apoptotic process involved in patterning of blood vessels [ISO]
- apoptotic signaling pathway [ISO, ISS]
- blood vessel remodeling [ISO]
- brain development [IEP]
- cell proliferation [ISO]
- cellular respiration [IDA]
- cellular response to DNA damage stimulus [ISO]
- cellular response to UV [ISO]
- cellular response to organic substance [ISO]
- cerebral cortex development [ISO]
- development of secondary sexual characteristics [ISO]
- ectopic germ cell programmed cell death [ISO]
- establishment or maintenance of transmembrane electrochemical gradient [ISO, ISS]
- extrinsic apoptotic signaling pathway [ISO]
- extrinsic apoptotic signaling pathway in absence of ligand [IBA, ISO]
- extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- fertilization [ISO]
- germ cell development [ISO]
- glial cell apoptotic process [IEP]
- glycosphingolipid metabolic process [ISO]
- homeostasis of number of cells [ISO]
- homeostasis of number of cells within a tissue [ISO]
- hypothalamus development [ISO]
- inner mitochondrial membrane organization [IDA]
- intrinsic apoptotic signaling pathway [ISO, ISS]
- intrinsic apoptotic signaling pathway by p53 class mediator [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage [IBA, ISO]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO, ISS]
- kidney development [ISO]
- leukocyte homeostasis [ISO]
- limb morphogenesis [ISO]
- male gonad development [ISO]
- mitochondrial fragmentation involved in apoptotic process [ISO, ISS]
- mitochondrial fusion [IBA, ISO, ISS]
- mitochondrion morphogenesis [ISO]
- myeloid cell homeostasis [ISO]
- negative regulation of apoptotic signaling pathway [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of endoplasmic reticulum calcium ion concentration [ISO]
- negative regulation of fibroblast proliferation [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of peptidyl-serine phosphorylation [ISO]
- negative regulation of protein binding [ISO, ISS]
- nervous system development [ISO]
- neuron apoptotic process [ISO]
- neuron migration [ISO]
- odontogenesis of dentin-containing tooth [ISO]
- outer mitochondrial membrane organization [IDA]
- ovarian follicle development [IEP, ISO]
- positive regulation of B cell apoptotic process [ISO]
- positive regulation of apoptotic DNA fragmentation [ISO]
- positive regulation of apoptotic process [IMP, ISO, ISS]
- positive regulation of apoptotic process involved in mammary gland involution [ISO]
- positive regulation of calcium ion transport into cytosol [ISO]
- positive regulation of developmental pigmentation [ISO]
- positive regulation of endoplasmic reticulum unfolded protein response [ISO, ISS]
- positive regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISO]
- positive regulation of intrinsic apoptotic signaling pathway [ISO, ISS]
- positive regulation of mitochondrial membrane permeability involved in apoptotic process [ISO]
- positive regulation of neuron apoptotic process [ISO, ISS]
- positive regulation of protein oligomerization [ISO, ISS]
- positive regulation of release of cytochrome c from mitochondria [ISO, ISS]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- post-embryonic camera-type eye morphogenesis [ISO]
- post-embryonic development [ISO]
- protein homooligomerization [ISO]
- protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [ISO]
- protein oligomerization [ISO]
- regulation of cell cycle [ISO]
- regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- regulation of mammary gland epithelial cell proliferation [ISO]
- regulation of mitochondrial membrane permeability involved in apoptotic process [ISO]
- regulation of mitochondrial membrane permeability involved in programmed necrotic cell death [ISO]
- regulation of mitochondrial membrane potential [ISO, ISS]
- regulation of neuron apoptotic process [ISO]
- regulation of nitrogen utilization [ISO]
- regulation of protein heterodimerization activity [ISO]
- regulation of protein homodimerization activity [ISO, ISS]
- release of cytochrome c from mitochondria [IBA, ISO]
- release of matrix enzymes from mitochondria [ISO, ISS]
- response to axon injury [ISO]
- response to cocaine [IEP]
- response to copper ion [IEP]
- response to corticosterone [IEP]
- response to drug [IEP]
- response to gamma radiation [ISO]
- response to ionizing radiation [ISO]
- response to salt stress [ISO]
- response to toxic substance [ISO, ISS]
- response to wounding [ISO]
- retina development in camera-type eye [ISO]
- retinal cell apoptotic process [ISO, ISS]
- retinal cell programmed cell death [ISO]
- sex differentiation [ISO]
- spermatid differentiation [ISO]
- spermatogenesis [ISO]
- thymocyte apoptotic process [ISO]
- transformed cell apoptotic process [ISO, ISS]
- vagina development [ISO]
Gene Ontology Molecular Function- BH domain binding [IPI]
- BH3 domain binding [ISO, ISS]
- channel activity [IBA, ISO, ISS]
- chaperone binding [IPI]
- heat shock protein binding [IPI]
- identical protein binding [ISO]
- lipid binding [ISO, ISS]
- protein binding [IPI]
- protein complex binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IBA, ISO]
- BH domain binding [IPI]
- BH3 domain binding [ISO, ISS]
- channel activity [IBA, ISO, ISS]
- chaperone binding [IPI]
- heat shock protein binding [IPI]
- identical protein binding [ISO]
- lipid binding [ISO, ISS]
- protein binding [IPI]
- protein complex binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IBA, ISO]
Gene Ontology Cellular Component
- BAX complex [ISO, ISS]
- Bcl-2 family protein complex [ISO, ISS]
- cell [ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO, ISS]
- extracellular vesicular exosome [ISO]
- intracellular [ISO]
- membrane [ISO]
- mitochondrial membrane [IDA, ISO]
- mitochondrial outer membrane [IDA, ISO]
- mitochondrial permeability transition pore complex [ISO, ISS]
- mitochondrion [IDA, ISO]
- nuclear envelope [ISO]
- nucleus [ISO, ISS]
- pore complex [ISO, ISS]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Bcl-2 changes conformation to inhibit Bax oligomerization.
Bcl-2 inhibits apoptosis by regulating the release of cytochrome c and other proteins from mitochondria. Oligomerization of Bax promotes cell death by permeabilizing the outer mitochondrial membrane. In transfected cells and isolated mitochondria, Bcl-2, but not the inactive point mutants Bcl-2-G145A and Bcl-2-V159D, undergoes a conformation change in the mitochondrial membrane in response to apoptotic agonists such as tBid and ... [more]
EMBO J. Jun. 07, 2006; 25(11);2287-96 [Pubmed: 16642033]
Throughput
- Low Throughput
Curated By
- BioGRID